Fully Automated Processing of Dried Blood Spot-Based Assays Using the MAP-C2 Workstation

Reuse of filter plates for elution of dried blood spot samples in neonatal assays.

Millipore MultiScreenTM filter plates are used for elution of dried blood spot samples in neonatal screening assays. A significant concern with the use of these plates is their cost. In this study we have evaluated the practice of reusing the filter plates in the Bio-Rad PKU and total galactose neonatal assays. All wells of the test plates used in the recovery study above were washed for a fift...

Estimation of blood phenylalanine from a dried blood spot using the Guthrie test.

Laser cutting eliminates nucleic acid cross-contamination in dried-blood-spot processing.

Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.

Measurement of Creatinine from Dried Blood spot by Enzymatic Method

Background: DBS on filter paper has been shown as a feasible matrix for collection, transport and analysis of biochemical analytes. Creatinine, is used as a marker of renal function and staging chronic kidney disease (CKD), often requiring repeated testing for drug dosing in patients who undergo dialysis or those who have had arenal transplant. Blood collected and dried on filter paper would be...

Stability of Proteins in Dried Blood Spot Biobanks*□S

An important motivation for the construction of biobanks is to discover biomarkers that identify diseases at early, potentially curable stages. This will require biobanks from large numbers of individuals, preferably sampled repeatedly, where the samples are collected and stored under conditions that preserve potential biomarkers. Dried blood samples are attractive for biobanking because of the...